Munzvimbo yesainzi yemazuva ano, protein electrophoresis inomira senzira yekona yekuongorora uye kuratidza mapuroteni.Pamwoyo wenzira iyi panePolyacrylamide, musanganiswa unosiyana-siyana unoshanda semusana wegel matrices anoshandiswa mumagetsi e electrophoresis systems.Polyacrylamide yakasarudzika zvimiro inoita kuti ive chishandiso chakakosha kune vanotsvaga uye masayendisiti ari kutsvaga kuburitsa kuomarara kweprotein nekudyidzana kwawo.
Polyacrylamide, inowanzonzi PAM, i polymer yakagadzirwa kubva kuacrylamide monomers.Kushanda kwakasiyana-siyana kunoshamisa kunokonzerwa nekukwanisa kwayo kuumba cheni dzakareba, zvichikonzera gel-senge chinhu chinogona kugarisa hukuru hwakasiyana hwemamorekuru.Ichi chivakwa chinoita kuti polyacrylamide ive yakakodzera mumiriri wekugadzira porous matrices anoshandiswa muprotein electrophoresis.
Protein electrophoresis inyanzvi inoparadzanisa mapuroteni zvichienderana nekuchaja kwavo uye saizi.Nekuisa sampu yeprotein kumunda wemagetsi mukati mepolyacrylamide gel matrix, mapuroteni anofamba nejeri pamitengo yakasiyana, zvichikonzera mabhendi akasiyana anogona kuongororwa uye kuverengerwa.Uku kupatsanurwa kunopa ruzivo rwakakosha mukuchena kweprotein, mamorekuru uremu kutsunga, uye kuvapo kweisoforms.
Basa rePolyacrylamide muProtein Electrophoresis
Sarudzo yepolyacrylamide yeprotein electrophoresis yakadzika midzi mune yayo inogoneka.Masayendisiti anogona kugadzirisa kusangana kweiyo gel matrix kuti igare mapuroteni ehukuru hwakasiyana.Kunyanya kukwira kunogadzira matrices akasimba akakodzera kugadzirisa mapuroteni madiki, nepo kudzika kwakadzika kuchishandiswa mapuroteni makuru.Uku kuchinjika kunovimbisa kuti vaongorori vanogona kugadzirisa zviedzo zvavo kuti vawane kupatsanurwa kwakanaka uye kuongororwa.
Polyacrylamide se aFlocculant
Polyacrylamide's utility inowedzera kupfuura basa rayo mugel electrophoresis.Iyo zvakare inowana zvikumbiro senge flocculant mumaindasitiri akasiyana, akadai sekuchenesa mvura uye kutambisa mvura.Sekuyerera, polyacrylamide inobatsira mukuunganidza zvakamiswa zvimedu muzvinwiwa, zvichiita kuti zvibviswe.Hunhu uhwu hunoratidza kugona kwakasiyana-siyana kwekomboni uye kukanganisa kwakasiyana-siyana pasainzi neindasitiri.
Kufambira mberi muPolyacrylamide-Based Electrophoresis
Makore achangopfuura akaona kuenderera mberi kwe polyacrylamide-based electrophoresis matekiniki.Native PEJI, SDS-PEJI, uye maviri-dimensional gel electrophoresis ingori mienzaniso mishoma yekuti kuchinjika kwepolyacrylamide kwakagonesa sei kugadzirwa kwenzira dzehunyanzvi dzekuongorora zvimiro zveprotein, post-translation shanduko, nekudyidzana.Aya matekiniki akakosha mukutsvaga kweproteomics uye kuedza kutsvaga zvinodhaka.
Munzvimbo yekuongorora mapuroteni, polyacrylamide inobuda seshamwari yakasimba, zvichiita kuti vaongorori vaongorore munyika yakaomarara yemapuroteni.Basa rayo senheyo yegel matrices mu electrophoresis systems haigoni kupfuudzwa.Kubva pakufumura nzira dzechirwere kusvika pakugadzira novel therapeutics, polyacrylamide-based electrophoresis inoenderera mberi nekuumba kufambira mberi kwesainzi.Sezvo tekinoroji iri kufambira mberi, ichi chinoshamisa chekugadzirwa chingangoshanduka, chichiwedzera kunzwisisa kwedu mapuroteni nemabasa awo mazhinji.
Nguva yekutumira: Aug-21-2023