Munyika yesainzi yemazuva ano, protein electrophoresis ndiyo nzira huru yekuongorora nekutsanangura mapuroteni. Pakati penzira iyi ndipo panePolyacrylamide, chinhu chinoshandiswa zvakasiyana-siyana chinoshanda semusana we gel matrices inoshandiswa muma gel electrophoresis systems. Hunhu hwakasiyana hwePolyacrylamide hunoita kuti ive chishandiso chakakosha kune vaongorori nemasayendisiti vari kutsvaga kutsanangura kuoma kwemapuroteni nekudyidzana kwawo.
Polyacrylamide, inowanzonzi PAM, ipolymer yakagadzirwa kubva kuacrylamide monomers. Kugona kwayo kuita zvinhu zvakasiyana-siyana kunokonzerwa nekukwanisa kwayo kugadzira cheni refu, zvichikonzera chinhu chakaita sejeli chinogona kugamuchira mamorekuru akasiyana-siyana. Izvi zvinoita kuti polyacrylamide ive yakakodzera kugadzira matrices ane maburi anoshandiswa mu protein electrophoresis.
Mapuroteni eelectrophoresis inzira inoparadzanisa mapuroteni zvichienderana nekuchaja kwawo nehukuru hwawo. Nekuisa sampuro yepuroteni mumunda wemagetsi mukati me polyacrylamide gel matrix, mapuroteni anofamba nepakati pe gel nematanho akasiyana, zvichikonzera mabhandi akasiyana anogona kuongororwa uye kuverengerwa. Kupatsanurwa uku kunopa ruzivo rwakakosha mukuchena kwepuroteni, kuona huremu hwemamorekuru, uye kuvapo kwe isoforms.
Basa rePolyacrylamide muProtein Electrophoresis
Kusarudzwa kwepolyacrylamide yeprotein electrophoresis kunobva mukugadziriswa kwayo. Masayendisiti anogona kugadzirisa huwandu hwegel matrix kuti ikwane mapuroteni ehukuru hwakasiyana. Huwandu hwakanyanya hunogadzira matrices dzakasimba dzakakodzera kugadzirisa mapuroteni madiki, nepo huwandu hwakaderera huchishandiswa kumapuroteni makuru. Kuchinjika uku kunoita kuti vaongorori vagone kugadzirisa zviedzo zvavo kuti vawane kupatsanurwa kwakanaka uye kuongororwa.
Polyacrylamide semushongaFlocculant
Kushanda kwePolyacrylamide hakupfuuri basa rayo mu gel electrophoresis. Inoshandiswawo se flocculant mumaindasitiri akasiyana-siyana, akadai sekurapa mvura uye kutarisira mvura yakasviba. Se flocculant, polyacrylamide inobatsira mukuunganidza zvidimbu zvakanyungudutswa mumvura, zvichiita kuti zvibviswe. Hunhu uhwu hunoratidza kugona kwakasiyana-siyana kwemusanganiswa uyu uye kukanganisa kwakakura kwesainzi nemaindasitiri.
Kufambira Mberi muPolyacrylamide-Based Electrophoresis
Makore achangopfuura akaona kufambira mberi kuri kuramba kuchiita muhunyanzvi hwe electrophoresis hwakavakirwa pa polyacrylamide. Native PAGE, SDS-PAGE, uye two-dimensional gel electrophoresis inongova mienzaniso mishoma yekuti kuchinjika kwe polyacrylamide kwakabatsira sei kuvandudzwa kwenzira dzakagadzirwa dzekuongorora maumbirwo emapuroteni, kugadziriswa kwemashure mekushandura, uye kudyidzana. Maitiro aya akakosha mukutsvaga kweproteomics uye kuedza kuwana mishonga.
Munyaya yekuongorora mapuroteni, polyacrylamide inobuda seshamwari yakasimba, zvichiita kuti vaongorori vakwanise kuongorora nyika yakaoma yemapuroteni. Basa rayo sehwaro hwema gel matrices muma electrophoresis systems harigone kupfuudzwa zvakanyanya. Kubva pakutsvaga nzira dzezvirwere kusvika pakugadzira mishonga mitsva, polyacrylamide-based electrophoresis inoramba ichiumba kufambira mberi kwesainzi. Sezvo tekinoroji ichifambira mberi, chishamiso ichi chekugadzira chingangochinja, zvichiwedzera kunzwisisa kwedu mapuroteni nemabasa awo akawanda.
Nguva yekutumira: Nyamavhuvhu-21-2023